The Fischer lab at St. Jude is recruiting a Scientist for a project that exploits the protein conformational landscape for ligand discovery in pediatric cancer. We are looking for applicants with a strong training in the gene-to-structure pipeline, including molecular biology, protein biochemistry, and structural biology (especially crystallography) and a keen interest or ideally experience in ligand discovery.
You will be part of an interdisciplinary team that uses modern structural biology (variable temperature crystallography) to characterize disease-relevant protein states, computational methods (MD, docking, custom software) to analyze and exploit them, and experiments (biophysical, biochemical, cell-based assays) to test the impact of small molecules on biology and disease. These approaches are also relevant for allosteric regulation of proteins and protein-protein interactions.
The successful candidate will have these attributes:
1) Must be able to conduct independent research in molecular biology and protein biochemistry, including cloning and protein purification, that enable structural studies.
2) Demonstrated experience in protein crystallography (from crystallization to data collection to refinement, analysis and deposition)
3) Familiarity with large-scale/ high-throughput approaches, automation, liquid handling, biophysical/ biochemical assays or computational approaches is desirable.
4) Experience with expression systems other than E. coli is a plus such as insect cells.
5) Proficiency in e.g. python or shell scripting will facilitate scaling up data analysis.
6) Must be eager to learn and implement new techniques from relevant areas.
Besides working on your own projects, you will form a liaison for other lab members and support projects within the lab while maintaining efficient laboratory operations.
Our group is housed in the Department of Chemical Biology and Therapeutics, and Structural Biology at St. Jude Children’s Research Hospital. With ongoing investments into exceptional structural and chemical biology facilities, this is an exciting time to join our lab to do cutting-edge research whilst living in an affordable city. Stable funding for this post is available.
Relevant papers include:
More info at: https://www.stjude.org/fischer